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Probing nucleic acid interactions and pre-mRNA splicing by Förster resonance energy transfer (FRET) microscopy
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SYSNO ASEP 0387290 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Probing nucleic acid interactions and pre-mRNA splicing by Förster resonance energy transfer (FRET) microscopy Author(s) Šimková, Eva (UMG-J)
Staněk, David (UMG-J) RIDSource Title International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 13, č. 11 (2012), s. 14929-14945Number of pages 17 s. Language eng - English Country US - United States Keywords FRET ; FLIM ; acceptor photobleaching ; RNA interactions ; spliceosome Subject RIV EB - Genetics ; Molecular Biology R&D Projects KAN200520801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Institutional support UMG-J - RVO:68378050 UT WOS 000311425000071 DOI https://doi.org/10.3390/ijms131114929 Annotation Förster resonance energy transfer (FRET) microscopy is a powerful technique routinely used to monitor interactions between biomolecules. Here, we focus on the techniques that are used for investigating the structure and interactions of nucleic acids (NAs). We present a brief overview of the most commonly used FRET microscopy techniques, their advantages and drawbacks. We list experimental approaches recently used for either in vitro or in vivo studies. Next, we summarize how FRET contributed to the understanding of pre-mRNA splicing and spliceosome assembly. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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