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The antimicrobial action of chitosan, low molar mass chitosan, and chitooligosaccharides on human colonic bacteria
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SYSNO ASEP 0387260 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The antimicrobial action of chitosan, low molar mass chitosan, and chitooligosaccharides on human colonic bacteria Author(s) Šimůnek, Jiří (UZFG-Y) RID
Brandysová, V. (CZ)
Koppová, Ingrid (UZFG-Y) RID
Šimůnek, Jiří Jr. (UZFG-Y)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 57, č. 4 (2012), 341-345Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords antibacterial activity ; intestinal bacteria ; in vitro Subject RIV EE - Microbiology, Virology R&D Projects GA525/08/0803 GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 UT WOS 000305980400020 DOI 10.1007/s12223-012-0138-1 Annotation Antibacterial effect of chitooligosaccharides (COS) and low molar mass chitosans (LMWC) is considered as one of the most important characteristics of chitosan (CS) hydrolysates. Here, we show the in vitro effect of different COS, LMWC, and CS on representative anaerobic bacteria isolated from human colon as a possibility of targeting modification of colonic microflora composition by supplementation of dietary CS products by humans. Specific growth rate of seven selected nonpathogenic anaerobic bacterial strains (Clostridium paraputrificum, Clostridium beijerinckii, Roseburia intestinalis, Bacteroides vulgatus, Bacteriodes thetaiotaomicron, Faecalibacterium prausnitzii and Blautia coccoides) was determined in the presence of 0.25 and 0.5% COS (2, 3, and 6 kDa), 0.025 and 0.05% of LMWC (10 and 16 kDa), and 0.025 and 0.1% of CS in vitro. The growth rate decreased in all strains in the presence of COS and LMWC in higher concentrations in comparison to control incubations. A relatively higher resistance to CS hydrolyzates was detected in R. intestinalis and F. prausnitzii, and more susceptible were bacteria belonging to Bacteoides sp. and Clostridium sp. The antimicrobial activity, minimum inhibitory concentrations (MIC), and minimal bactericidal concentrations (MBC) were determined. The antimicrobial activity increased with the degree of polymerization (DP). MIC ranged from 0.25 to 4.5% in dependence on bacterial strain and DP of CS/LMWC. MBC also decreased with DP. The most effective antimicrobial action was detected in LMWC with 16 kDa and CS. Weak antimicrobial activity was found in COS with small molecules (2 and 3 kDa). Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2013
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