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Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma
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SYSNO ASEP 0387101 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Surface plasmon resonance biosensor based on engineered proteins for direct detection of interferon-gamma in diluted blood plasma Author(s) Šípová, Hana (URE-Y)
Ševců, Veronika (URE-Y)
Kuchař, Milan (BTO-N) RID, ORCID
Ahmad, Jawid Nazir (MBU-M) RID
Mikulecký, Pavel (BTO-N) RID
Osičková, Adriana (MBU-M) RID, ORCID
Malý, Petr (BTO-N) RID, ORCID
Homola, Jiří (URE-Y) RIDNumber of authors 8 Source Title Sensors and Actuators B - Chemical. - : Elsevier - ISSN 0925-4005
Roč. 174, č. 11 (2012), s. 306-311Number of pages 6 s. Language eng - English Country CH - Switzerland Keywords Interferon gamma ; Surface plasmon resonance ; Biosensor Subject RIV JB - Sensors, Measurment, Regulation R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Institutional support URE-Y - RVO:67985882 ; MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 UT WOS 000311329000044 DOI https://doi.org/10.1016/j.snb.2012.08.024 Annotation We present a novel surface plasmon resonance (SPR) biosensor for direct detection of human interferon gamma (hIFN). The biorecognition elements for hIFNare engineered proteins derived from albumin binding domain (ABD). Two methods for the immobilization of ABD are compared: covalent coupling and immobilization via streptavidin–biotin interaction. The developed biosensor provides rapid and sensitive platform for the detection of hIFN at subnanomolar levels both in buffer and diluted human plasma. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2013
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