Iron oxide nanoparticle modified monolithic pipette tips for selective enrichment of phosphopeptides

0386147 - ÚIACH 2013 RIV CZ eng C - Conference Paper (international conference)
Křenková, Jana - Foret, František
Iron oxide nanoparticle modified monolithic pipette tips for selective enrichment of phosphopeptides.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 349-351. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
R&D Projects: GA ČR(CZ) GAP301/11/2055; GA ČR(CZ) GBP206/12/G014
Grant - others:Jihomoravský kraj(CZ) 2SGA2721
Program: 2SGA
Institutional support: RVO:68081715
Keywords : iron oxide nanoparticles * monolith * phosphopeptide enrichment
Subject RIV: CB - Analytical Chemistry, Separation

We have developed iron oxide nanoparticle modified monolithic pipette tips for selective and efficient enrichment of phosphopeptides. Iron oxide nanoparticles were synthesized using a co-precipitation method and stabilized by citrate ions. A stable coating of nanoparticles was obtained via multivalent interactions of citrate ions on the nanoparticle surface with a quaternary amine functionalized surface of the methacrylate based monolithic tips. The performance of the developed and commercially available tips was demonstrated with the enrichment of phosphorylated peptides from tryptic digests of caseins followed by MALDI/MS detection.
Permanent Link: http://hdl.handle.net/11104/0215394