The efficient approaches for enzymatic digestion of plant proteins

0386005 - ÚIACH 2013 RIV CZ eng C - Conference Paper (international conference)
Dyčka, Filip - Bobáľ, P. - Bobálová, Janette
The efficient approaches for enzymatic digestion of plant proteins.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 267-270. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
R&D Projects: GA MŠMT 1M0570; GA MŠMT 1M06030; GA MŠMT(CZ) EE2.3.20.0182
Institutional support: RVO:68081715
Keywords : ultrasonic * infrared * digestion
Subject RIV: CB - Analytical Chemistry, Separation

In this work the fast and efficient methods applying ultrasound and infrared radiation were employed for in-gel digestion of proteins extracted from Hordeum vulgare. These techniques can be used instead of time consuming conventional protocol prior mass spectrometry analysis. The comparative study indicates that the examined protocols are able to enhance digestion efficiency and protein identification.
Permanent Link: http://hdl.handle.net/11104/0215222