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Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells
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SYSNO ASEP 0384624 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mechanisms of natural brassinosteroid-induced apoptosis of prostate cancer cells Author(s) Steigerová, J. (CZ)
Rárová, L. (CZ)
Oklešťková, Jana (UEB-Q) RID, ORCID, SAI
Křížová, K. (CZ)
Levková, M. (CZ)
Šváchová, M. (CZ)
Kolář, Z. (CZ)
Strnad, Miroslav (UEB-Q) RID, ORCIDSource Title Food and Chemical Toxicology. - : Elsevier - ISSN 0278-6915
Roč. 50, č. 11 (2012), s. 4068-4076Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords Apoptosis ; Brassinosteroids ; Cell cycle Subject RIV FD - Oncology ; Hematology R&D Projects GA301/08/1649 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000310654300027 DOI https://doi.org/10.1016/j.fct.2012.08.031 Annotation Brassinosteroids (BRs) are a group of polyhydroxylated sterol derivatives with important regulatory roles in various plant physiological processes. The aim of this study was to examine the mechanism of the anti-proliferative activity of natural BRs 28-homocastasterone (28-homoCS) and 24-epibrassinolide (24-epiBL) in hormone-sensitive and -insensitive (LNCaP and DU-145, respectively) human prostate cancer cell lines. The effects of BRs on prostate cancer cells were surveyed using flow cytometry, Western blotting, TUNEL, DNA ladder assays and immunofluorescence analyses. The studied BRs inhibited cell growth and induced G(1) blocks in LNCaP cells accompanied by reductions in cyclin D-1, CDK4/6 and pRb expression. Following BR treatment of DU-145 cells, increases in proportions of cells in the G(2)/M phase of cell cycle were observed, accompanied by down-regulation of cyclins A and B-1. Changes in AR localization patterns in LNCaP cells treated with BRs were shown by immunofluorescence analysis. Furthermore, apoptotic detection methods demonstrated induction of apoptosis mediated by BRs in both cell lines, although changes in the expression of apoptosis-related proteins were modulated differently by 28-homoCS and 24-piBL in each cell line. The studied BRs seem to exert potent growth inhibitory and pro-apoptotic effects and could be therefore highly valuable new candidates for prostate anticancer drugs. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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