0384593 - ÚOCHB 2013 RIV CH eng J - Journal Article
Brabcová, Jana - Blažek, Jiří - Janská, Lucie - Krečmerová, Marcela - Zarevúcka, Marie
Lipases as Tools in the Synthesis of Prodrugs from Racemic 9-(2,3-Dihydroxypropyl)adenine.
Molecules. Roč. 17, č. 12 (2012), s. 13813-13824. ISSN 1420-3049. E-ISSN 1420-3049
Grant - others:AV ČR(CZ) M200551203
Institutional support: RVO:61388963
Keywords : lipase * transesterification * prodrug * immobilization of enzymes
Subject RIV: CC - Organic Chemistry
Impact factor: 2.428, year: 2012 ; AIS: 0.573, rok: 2012
DOI: https://doi.org/10.3390/molecules171213813

Lipases from Geotrichum candidum 4013 (extracellular lipase and cell-bound lipase) were immobilized by adsorption on chitosan beads. The enzyme preparations were tested in the synthesis of ester prodrugs from racemic 9-(2,3-dihydroxypropyl)adenine in dimethylformamide with different vinyl esters (acetate, butyrate, decanoate, laurate, palmitate). The transesterification activities of these immobilized enzymes were compared with commercially available lipases (lipase from hog pancreas, Aspergillus niger, Candida antarctica, Pseudomonas fluorescens).
Permanent Link: http://hdl.handle.net/11104/0214185