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Selected Ion Flow Tube-MS Analysis of Headspace Vapor from Gastric Content for the Diagnosis of Gastro-Esophageal Cancer
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SYSNO ASEP 0384534 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Selected Ion Flow Tube-MS Analysis of Headspace Vapor from Gastric Content for the Diagnosis of Gastro-Esophageal Cancer Author(s) Kumar, S. (GB)
Huang, J. (GB)
Cushnir, J. R. (GB)
Španěl, Patrik (UFCH-W) RID, ORCID
Smith, D. (GB)Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 84, č. 21 (2012), s. 9550-9557Number of pages 8 s. Language eng - English Country US - United States Keywords SOLID-PHASE MICROEXTRACTION ; TRACE GAS-ANALYSIS ; CHROMATOGRAPHY-MASS SPECTROMETRY Subject RIV CF - Physical ; Theoretical Chemistry Institutional support UFCH-W - RVO:61388955 UT WOS 000310664600094 DOI https://doi.org/10.1021/ac302409a Annotation Gastric content is a complex biofluid within the human stomach which has an important role in digestive processes. It is believed that gastric content may be a contributory factor in the development of upper gastro-intestinal diseases. In this work, selected ion flow tube mass spectrometry (SIFT-MS) has been applied to the quantification of volatile organic compounds (VOCs) in the headspace vapor of gastric content samples, which were retrieved from three groups of patients, including those with gastro-esophageal cancer, noncancer diseases of the upper gastro-intestinal tract, and a healthy cohort. Twelve VOCs have been investigated in this study;. the following 7 VOCs, acetone, formaldehyde, acetaldehyde, hexanoic acid, hydrogen sulphide, hydrogen cyanide, and methyl phenol, were found to be significantly different between cancer and healthy groups by the Mann-Whitney U test. Receiver operating characteristics (ROC) analysis was applied for the combined VOCs of acetaldehyde, formaldehyde, hydrogen sulphide, and methyl phenol to discriminate cancer patients from healthy controls. The area under the curve (AUC) was 0.9. This result raises the prospect that a VOC profile rather than a single biomarker may be preferable in the molecular-orientated diagnosis of gastro-oseophageal cancer, and this warrants further investigation to assess its potential application as a new diagnostic test. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2013
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