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Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions
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SYSNO ASEP 0384160 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions Author(s) Gilchrist, F. J. (GB)
Sims, H. (GB)
Alcock, A. (GB)
Belcher, J. (GB)
Jones, A.M. (GB)
Smith, D. (GB)
Španěl, Patrik (UFCH-W) RID, ORCID
Webb, A. K. (GB)
Lenney, W. (GB)Source Title Analytical Methods: advancing methods and applications . - : Royal Society of Chemistry - ISSN 1759-9660
Roč. 4, č. 11 (2012), s. 3661-3665Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords tube mass spectrometry ; cystic fibrosis ; volatile metabolites Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA203/09/0256 GA ČR - Czech Science Foundation (CSF) Institutional support UFCH-W - RVO:61388955 UT WOS 000310281700025 DOI https://doi.org/10.1039/c2ay25652e Annotation Hydrogen cyanide (HCN) and 2-aminoacetophenone (2-AA; H2NC6H4COCH3) are possible biomarkers of pulmonary Pseudomonas aeruginosa (PA) infection that could be used in an exhaled breath test. All factors affecting their production need to be investigated, including the culture conditions: planktonic (free-floating) or biofilm (non-motile communities attached to a solid surface). In vivo, the change from planktonic to biofilm growth is signalled when a certain population density is reached. Using selected ion flow tube mass spectrometry, SIFT-MS, we have analysed HCN and 2-AA produced by 12 genotyped PA samples, cultured under both planktonic and biofilm conditions after 24, 48, 72 and 96 hours of incubation. The 12 samples included 3 different strains (genotypes), 50% of which had a mucoid phenotype and 50% had a non-mucoid phenotype. All samples produced significant concentrations of HCN; median (25th to 75th percentiles, IQR) concentration: 144 (61-512) parts-per-billion by volume (ppbv). Multivariate analysis showed HCN production varied dependent on genotype (p = 0.0014), culture duration (p = 0.005) and phenotype (p < 0.001) but not culture conditions (planktonic/biofilm). Much smaller concentrations of 2-AA were detected, median (IQR) concentration 1.8 (1.3-3) ppbv, despite which, multivariate analysis showed production was affected by genotype (p < 0.001) and culture duration (p = 0.007) but not phenotype or culture conditions. These data show that biofilm formation does not affect HCN production by PA and supports its use as a biomarker of PA infection. The concentrations of 2-AA are much lower than previous studies have shown. The reason for this is unclear but it raises questions about its suitability as a biomarker of PA infection. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2013
Number of the records: 1