0384090 - ÚPT 2013 RIV US eng J - Journal Article
Neděla, Vilém - Hřib, J. - Svidenská, S. - Vooková, B. - Runštuk, Jiří
Environmental Scanning Electron Microscope As A Tool For Imaging Of Native State Somatic Embryogenesis.
Microscopy and Microanalysis. Roč. 18, Suppl. 2 (2012), s. 1270-1271. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA ČR GAP102/10/1410; GA MPO FR-TI1/305; GA MPO FR-TI1/118; GA MŠMT EE.2.3.20.0103
Institutional support: RVO:68081731
Keywords : environmental scanning electron microscopy * somatic embryogenesis
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 2.495, year: 2012 ; AIS: 1.286, rok: 2012
DOI: https://doi.org/10.1017/S1431927612008203

The development of environmental scanning electron microscopy (ESEM) was originally driven by a growing need to overcome the limitations of the conventional SEM in order to facilitate the research and characterization of a greater range of specimen types and expand the available experimental methodologies. In the environmental scanning electron microscope (ESEM) the specimen, can be observed across a wide range of pressure from the vacuum (comparable with the SEM) to the high pressure of various gases (over a thousand Pa) in the specimen chamber. If the pressure of the gas is sufficiently increased or the temperature of the samples reduced, very wet non-conductive samples can be observed in their natural state and free of charging artefacts.
Permanent Link: http://hdl.handle.net/11104/0217617