Number of the records: 1
Re-evaluating the green versus red signal in eukaryotes with secondary plastid of red algal origin
- 1.
SYSNO ASEP 0382165 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Re-evaluating the green versus red signal in eukaryotes with secondary plastid of red algal origin Author(s) Burki, F. (CA)
Flegontov, Pavel (BC-A) RID, ORCID
Oborník, Miroslav (BC-A) RID, ORCID
Cihlář, Jaromír (BC-A) SAI
Pain, A. (SA)
Lukeš, Julius (BC-A) RID, ORCID
Keeling, P. J. (CA)Source Title Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
Roč. 4, č. 6 (2012), s. 738-747Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords Endosymbiotic gene transfer ; plastid evolution ; protist ; algae ; chromera Subject RIV EB - Genetics ; Molecular Biology Subject RIV - cooperation Institute of Microbiology R&D Projects GAP506/12/1522 GA ČR - Czech Science Foundation (CSF) GBP501/12/G055 GA ČR - Czech Science Foundation (CSF) Institutional support BC-A - RVO:60077344 UT WOS 000313211300001 DOI https://doi.org/10.1093/gbe/evs049 Annotation The transition from endosymbiont to organelle in eukaryotic cells involves the transfer of significant numbers of genes to the host genomes, a process known as endosymbiotic gene transfer (EGT). In the case of plastid organelles, EGTs have been shown to leave a footprint in the nuclear genome that can be indicative of ancient photosynthetic activity in present-day plastid-lacking organisms, or even hint at the existence of cryptic plastids. Here, we evaluated the impact of EGT on eukaryote genomes by reanalyzing the recently published EST dataset for Chromera velia, an interesting test case of a photosynthetic alga closely related to apicomplexan parasites. Previously, 513 genes were reported to originate from red and green algae in a 1:1 ratio. In contrast, by manually inspecting newly generated trees indicating putative algal ancestry, we recovered only 51 genes congruent with EGT, of which 23 and 9 were of red and green algal origin, respectively, whereas 19 were ambiguous regarding the algal provenance. Our approach also uncovered 109 genes that branched within a monocot angiosperm clade, most likely representing a contamination. We emphasize the lack of congruence and the subjectivity resulting from independent phylogenomic screens for EGT, which appear to call for extreme caution when drawing conclusions for major evolutionary events. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2013
Number of the records: 1