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NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases
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SYSNO ASEP 0381547 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title NADPH oxidase activity in pollen tubes is affected by calcium ions, signaling phospholipids and Rac/Rop GTPases Author(s) Potocký, Martin (UEB-Q) RID, ORCID
Pejchar, Přemysl (UEB-Q) RID, ORCID
Gutkowska, Malgorzata (UEB-Q)
Jiménez-Quesada, M. J. (ES)
Potocká, Andrea (UEB-Q) RID
Alché, J. (ES)
Kost, B. (DE)
Žárský, Viktor (UEB-Q) RID, ORCIDSource Title Journal of Plant Physiology. - : Elsevier - ISSN 0176-1617
Roč. 169, č. 16 (2012), s. 1654-1663Number of pages 10 s. Language eng - English Country PT - Portugal Keywords Pollen tube ; Tip growth ; NADPH oxidase Subject RIV EB - Genetics ; Molecular Biology R&D Projects GP522/09/P299 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000311763200010 DOI https://doi.org/10.1016/j.jplph.2012.05.014 Annotation Reactive oxygen species (ROS) generated by NADPH oxidase (NOX) are crucial for tip growth of pollen tubes. However, the regulation of NOX activity in pollen tubes remains unknown. Using purified plasma membrane fractions from tobacco and olive pollen and tobacco BY-2 cells, we demonstrate that pollen NOX is activated by calcium ions and low abundant signaling phospholipids, such as phosphatidic acid and phosphatidylinositol 4,5-bisphosphate in vitro and in vivo. Our data also suggest possible synergism between Ca2+ and phospholipid-mediated NOX activation in pollen. Rac/Rop small GTPases are also necessary for normal pollen tube growth and have been proposed to regulate ROS production in root hairs. We show here elevated ROS formation in pollen tubes overexpressing wild-type NtRac5 and constitutively active NtRac5, while overexpression of dominant-negative NtRac5 led to a decrease of ROS in pollen tubes. We also show that PA formed by distinct phospholipases D (PLD) is involved in pathways both upstream and downstream of NOX-mediated ROS generation and identify NtPLDδ as a PLD isoform acting in the ROS response pathway. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2013
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