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Microsatellite distribution on sex chromosomes at different stages of heteromorphism and heterochromatinization in two lizard species (Squamata: Eublepharidae: Coleonyx elegans and Lacertidae: Eremias velox)
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SYSNO ASEP 0381524 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microsatellite distribution on sex chromosomes at different stages of heteromorphism and heterochromatinization in two lizard species (Squamata: Eublepharidae: Coleonyx elegans and Lacertidae: Eremias velox) Author(s) Pokorná, Martina (UZFG-Y) RID, ORCID
Kratochvíl, L. (CZ)
Kejnovský, Eduard (BFU-R) RID, ORCIDSource Title B M C Genetics - ISSN 1471-2156
Roč. 12, č. 1 (2011), s. 90-96Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords repeated dna-sequences ; Y-chromosome ; determining mechanisms Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP506/10/0718 GA ČR - Czech Science Foundation (CSF) GAP305/10/0930 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) AV0Z50040702 - BFU-R (2007-2013) UT WOS 000296964400001 DOI 10.1186/1471-2156-12-90 Annotation The accumulation of repetitive sequences such as microsatellites during the differentiation of sex chromosomes has not been studied in most squamate reptiles (lizards, amphisbaenians and snakes), a group which has a large diversity of sex determining systems. It is known that the Bkm repeats containing tandem arrays of GATA tetranucleotides are highly accumulated on the degenerated W chromosomes in advanced snakes. Similar, potentially homologous, repetitive sequences were found on sex chromosomes in other vertebrates. Using FISH with probes containing all possible mono-, di-, and tri-nucleotide sequences and GATA, we studied the genome distribution of microsatellite repeats on sex chromosomes in two lizard species (the gecko Coleonyx elegans and the lacertid Eremias velox) with independently evolved sex chromosomes. The gecko possesses heteromorphic euchromatic sex chromosomes, while sex chromosomes in the lacertid are homomorphic and the W chromosome is highly heterochromatic. Our aim was to test whether microsatellite distribution on sex chromosomes corresponds to the stage of their heteromorphism or heterochromatinization. Moreover, because the lizards lie phylogenetically between snakes and other vertebrates with the Bkm-related repeats on sex chromosomes, the knowledge of their repetitive sequence is informative for the determination of conserved versus convergently evolved repetitive sequences across vertebrate lineages. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2013
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