0381380 - BÚ 2013 RIV US eng J - Journal Article
Přibyl, Pavel - Eliáš, M. - Cepák, Vladislav - Lukavský, Jaromír - Kaštánek, P.
Zoosporogenesis, morphology, ultrastructure, pigment composition, and phylogenetic position of Trachydiscus minutus (Eustigmatophyceae, Heterokontophyta).
Journal of Phycology. Roč. 48, č. 1 (2012), s. 231-242. ISSN 0022-3646. E-ISSN 1529-8817
R&D Projects: GA MŠMT 1M0571; GA ČR GPP501/10/P157
Institutional research plan: CEZ:AV0Z60050516
Keywords : phylogenetics * Trachydiscus * zoospores
Subject RIV: EF - Botanics
Impact factor: 2.239, year: 2012 ; AIS: 0.76, rok: 2012
DOI: https://doi.org/10.1111/j.1529-8817.2011.01109.x

We discovered a zoosporic part of the life cycle of the microalga Trachydiscus minutus, strain CCALA 838 and described conditions affecting zoosporogenesis and morphology of zoospores. Ultrastructural investigations of vegetative cells revealed plastids lacking both a connection to the nuclear envelope and a girdle lamella. Moreover, we described biogenesis of oil bodies on the ultrastructural level. Photosynthetic pigments of T. minutus included as the major carotenoids violaxanthin and vaucheriaxanthin (ester); we detected no chl c. An 18S rRNA gene-based phylogenetic analysis placed T. minutus in a clade with species of the genus Pseudostaurastrum and with Goniochloris sculpta, which form a sister branch to initially studied Eustigmatophyceae. In summary, our results are inconsistent with classifying T. minutus as a xanthophycean and indicate that it is a member of a novel deep lineage of the class Eustigmatophyceae.
Permanent Link: http://hdl.handle.net/11104/0211866