0381075 - BÚ 2013 RIV US eng J - Journal Article
Krak, Karol - Álvarez, I. - Caklová, Petra - Costa, A. - Chrtek, Jindřich - Fehrer, Judith
Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes.
American Journal of Botany. Roč. 99, č. 2 (2012), s. 74-77. ISSN 0002-9122. E-ISSN 1537-2197
R&D Projects: GA ČR GAP506/10/1363; GA ČR GA206/05/0657
Institutional research plan: CEZ:AV0Z60050516
Keywords : Asteraceae * Hieraciinae * low-copy nuclear markers
Subject RIV: EF - Botanics
Impact factor: 2.586, year: 2012 ; AIS: 1.087, rok: 2012
DOI: https://doi.org/10.3732/ajb.1100416

The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with public DNA sequence databases. Interspecifi c variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for other Asteraceae tribes. All three markers had higher variation (2.1 − 4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplifi cation was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, especially for very closely related species.
Permanent Link: http://hdl.handle.net/11104/0211623