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Characteristics of bovine oocytes with different meiotic competence in terms of their mitochondrial status and expression of nuclear-encoded factors
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SYSNO ASEP 0380415 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characteristics of bovine oocytes with different meiotic competence in terms of their mitochondrial status and expression of nuclear-encoded factors Author(s) Machatková, M. (CZ)
Jeseta, M. (CZ)
Hulínská, P. (CZ)
Knitlová, D. (CZ)
Němcová, Lucie (UZFG-Y) RID
Kaňka, Jiří (UZFG-Y) RID, ORCIDNumber of authors 6 Source Title Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768
Roč. 47, č. 5 (2012), s. 806-814Number of pages 9 s. Language eng - English Country DE - Germany Keywords bovine oocytes ; mitochondrial status ; nuclear-encoded factors Subject RIV GI - Animal Husbandry ; Breeding R&D Projects QI91A018 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000308087900020 DOI 10.1111/j.1439-0531.2011.01972.x Annotation The present study was designed to characterize bovine oocytes with different meiotic competence and atresia levels in terms of their mitochondrial status. Oocyte subpopulations were recovered either from medium (MF) or small (SF) follicles and categorized as healthy, light-atretic and mid-atretic according to oocyte morphology. Mitochondrial activity, morphology and distribution, adenosine triphosphate (ATP) content and expression of mitochondrial transcription factor A (TFAM) and nuclear respiratory factor 1 (NRF1) were assessed before (GV) and after (MII) maturation. The data were related to follicular size regardless of or with regard to oocyte atresia. Regardless of atresia, the MF subpopulation showed a significantly higher mitochondrial activity and frequency of oocytes with granulated mitochondria at GV and clustered mitochondria at MII than the SF subpopulation. With regard to atresia, mitochondrial activity decreased from healthy to mid-atretic oocytes in both MF and SF subpopulations at GV, but in the SF subpopulation at MII, the mitochondrial activity and frequency of oocytes with clustered mitochondria were significantly higher in light-atretic than in healthy oocytes. The light-atretic oocytes also produced more ATP than healthy ones in both SF and MF subpopulations. However, a significantly higher relative abundance of mRNA TFAM was found in SF than MF subpopulations at GV, and this difference remained in mid-atretic oocytes at MII. It can be concluded that meiotic competence and atresia level influence mitochondrial status of immature bovine oocytes. After maturation, healthy oocytes from medium follicles and light-atretic oocytes from small follicles were more developed in terms of mitochondrial status than the other oocytes. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2013
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