Number of the records: 1
Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP
- 1.
SYSNO ASEP 0379796 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Enhancement of immune response towards non-lipidized Borrelia burgdorferi recombinant OspC antigen by binding onto the surface of metallochelating nanoliposomes with entrapped lipophilic derivatives of norAbuMDP Author(s) Křupka, M. (CZ)
Mašek, J. (CZ)
Bartheldyová, E. (CZ)
Turánek Knotigová, P. (CZ)
Plocková, J. (CZ)
Korvasová, Z. (CZ)
Škrabalová, M. (CZ)
Koudelka, Š. (CZ)
Kulich, P. (CZ)
Zachová, K. (CZ)
Czerneková, L. (CZ)
Strouhal, O. (CZ)
Horynová, M. (CZ)
Šebela, M. (CZ)
Miller, A. D. (GB)
Ledvina, Miroslav (UOCHB-X) RID
Raška, M. (CZ)
Turánek, J. (CZ)Number of authors 18 Source Title Journal of Controlled Release. - : Elsevier - ISSN 0168-3659
Roč. 160, č. 2 (2012), s. 374-381Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords Borrelia burgdorferi ; OspC ; metallochelating liposomes ; MDP Subject RIV CC - Organic Chemistry R&D Projects GAP304/10/1951 GA ČR - Czech Science Foundation (CSF) TA01011165 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) KAN200520703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000305788800033 DOI https://doi.org/10.1016/j.jconrel.2012.02.017 Annotation Lyme disease caused by spirochete Borrelia burgdorferi sensu lato, is a tick-born illness. If the infection is not eliminated by the host immune system and/or antibiotics, it may further disseminate and cause severe chronic complications. The immune response to Borrelia is mediated by phagocytic cells and by Borrelia-specific complement-activating antibodies associated with Th1 cell activation. A new experimental vaccine was constructed using non-lipidized form of recombinant B. burgdorferi s.s. OspC protein was anchored by metallochelating bond onto the surface of nanoliposomes containing novel nonpyrogenic normuramyglycopeptides modified at carboxy terminus of the peptide part of their molecules by bulky lipophilic acyl residue. After i.d. immunization, the experimental vaccines surpassed Alum with respect to OspC-specific titers of IgG2a, IgG2b isotypes when analog modified with branch fatty acid was used and IgG3, IgM isotypes when analog modified with linear fatty acid was used. Both adjuvants exerted a high adjuvant effect comparable or better than MDP and proved themselves as nonpyrogenic. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2013
Number of the records: 1