Preferential binding of IFI16 protein to cruciform structure and superhelical DNA

0379230 - BFÚ 2013 RIV US eng J - Journal Article
Brázda, Václav - Coufal, Jan - Liao, J.C.C. - Arrowsmith, Ch.H.
Preferential binding of IFI16 protein to cruciform structure and superhelical DNA.
Biochemical and Biophysical Research Communications. Roč. 422, č. 4 (2012), s. 716-720. ISSN 0006-291X. E-ISSN 1090-2104
R&D Projects: GA ČR(CZ) GAP301/10/1211
Institutional research plan: CEZ:AV0Z50040702
Keywords : cruciform structure * IFI16 * protein-DNA binding
Subject RIV: BO - Biophysics
Impact factor: 2.406, year: 2012

Interferon (IFN)-inducible HIN-200 proteins play an important role in transcriptional regulation linked to cell cycle control, inflammation, autoimmunity and differentiation. We demonstrate that IFI16 protein binds strongly to negatively superhelical plasmid DNA. We also detect a strong preference for IFI16 binding to cruciform DNA structure compared to linear DNA topology. This novel DNA-binding property of IFI16 protein to scDNA and cruciform structures may play critical roles in its tumor suppressor function.
Permanent Link: http://hdl.handle.net/11104/0210485