Number of the records: 1
Recombinant fragment of an antibody tailored for direct radioiodination
- 1.
SYSNO ASEP 0378891 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Recombinant fragment of an antibody tailored for direct radioiodination Author(s) Sedláček, Juraj (UMG-J) RID
Fábry, Milan (UMG-J) RID
Sieglová, Irena (UMG-J)
Král, Vlastimil (UMG-J) RID
Uhnáková, Bronislava (UMG-J)
Mudra, M. (SK)
Kronrád, L. (CZ)
Sawicka, A. (PL)
Mikolajczak, R. (PL)
Řezáčová, Pavlína (UMG-J) RIDSource Title Journal of Labelled Compounds and Radiopharmaceuticals. - : Wiley - ISSN 0362-4803
Roč. 55, č. 1 (2012), s. 52-56Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords I125 labelling ; single-chain antibody variable fragment ; tyrosine-rich polypeptide segment ; fusion protein Subject RIV EB - Genetics ; Molecular Biology R&D Projects 2A-2TP1/076 GA MPO - Ministry of Industry and Trade (MPO) 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000299555700010 DOI 10.1002/jlcr.1945 Annotation High specific radioactivity (per mass or per mole) is an important and desirable parameter for the radiopharmaceuticals that are intended to target disease biomarker molecules. In direct radioiodination of immunoglobulins or their fragments, the predominant chemical reaction is usually the monoiodination of the phenol rings of tyrosine amino acid residues. For such radioiodination, the maximum achievable level of labeling of a given protein molecule obviously correlates to the number of solvent-accessible tyrosine residues. The grafting of proteins with additional iodine-acceptor tyrosine residues by conventional chemical techniques has been recently reported. We show here that a recombinant single chain variable fragment (scFv) of an anti-b-III-tubulin antibody, having a potential use in imaging neural lesions or astrocytoma and certain nonneuronal tumors, can be modified in a similar manner. However, in contrast to previously published examples, tyrosine residues are added to the polypeptide main chain without any chemical reaction, solely with molecular cloning and recombinant expression. Importantly, the tailoring outlined here is carried out ‘once and for all’ at laboratory scale (modifying permanently the expressed nucleic acid), whereas chemical indirect methods are necessarily carried out either at production scale or always before each round of radioiodination. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
Number of the records: 1