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3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging
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SYSNO ASEP 0373200 Document Type O - Others R&D Document Type Others Title 3D visualization of collagen in artificial scaffolds using two-photon excitation and second-harmonic generation imaging Author(s) Čapek, M. (CZ)
Burdíková, Zuzana (FGU-C) RID
Filová, Eva (UEM-P) RID, ORCID
Košťáková, E. (CZ)
Janáček, Jiří (FGU-C) RID, ORCIDSource Title Proceedings 10th Multinational Congress on Microscopy 2011. - Urbino : Societa Italiana Scienze Micriscopiche, 2011
S. 261-262Number of pages 2 s. Action Multinational Congress on Microscopy 2011 /10./ - MCM 2011 Event date 04.09.2011-09.09.2011 VEvent location Urbino Country IT - Italy Event type WRD Language eng - English Country IT - Italy Keywords multi-photon microscopy ; artificial scaffolds ; collagen Subject RIV EA - Cell Biology R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) MEB0810092 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP501/10/0340 GA ČR - Czech Science Foundation (CSF) GA304/09/0733 GA ČR - Czech Science Foundation (CSF) IAA500390702 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 7E09088 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50110509 - FGU-C (2005-2011) AV0Z50390512 - UEM-P (2005-2011) Annotation Nonlinear microscopy can employ two different types of signals, fluorescence and second-harmonic generation (SHG), to image biological structures with subcellular resolution. SHG imaging was used for the identification of type II collagen in an extracellular matrix. Chondrocyte proliferation was linked to production of the extracellular matrix proteins. Collagen as a typical extracellular cartilage protein was visualized, first, by CLSM using fluorescently labeled monoclonal antibodies against type II collagen. Conventional confocal microscopy images were compared with the SHG imaging in both forward and backward nondescanned modes. Well detectable signals were observed in PCL and gelatine scaffolds. Detection of collagen by SHG imaging corresponded well with collagen detection by immunohistochemical staining Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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