Recognition of peptidoglycan and beta-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae

Maestro, B.; Nováková, Linda; Hesek, D.; Lee, M.; Leyva, E.; Mobashery, S.; Sanz, J.M.; Branny, Pavel

doi:https://dx.doi.org/10.1016/j.febslet.2010.12.016

Number of the records: 1

Recognition of peptidoglycan and beta-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae

1.

SYSNO ASEP	0370932
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Recognition of peptidoglycan and beta-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae
Author(s)	Maestro, B. (ES) Nováková, Linda (MBU-M)_RID Hesek, D. (US) Lee, M. (US) Leyva, E. (ES) Mobashery, S. (US) Sanz, J.M. (ES) Branny, Pavel (MBU-M)_{RID, ORCID}
Source Title	FEBS Letters. - : Wiley - ISSN 0014-5793 Roč. 585, č. 2 (2011), s. 357-363
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Signal transduction ; Penicillin-binding protein and Ser/Thr protein kinase-associated domain ; Peptidoglycan
Subject RIV	CE - Biochemistry
R&D Projects	GP204/07/P082 GA ČR - Czech Science Foundation (CSF)
	GA204/08/0783 GA ČR - Czech Science Foundation (CSF)
	IAA600200801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000286180300016
DOI	10.1016/j.febslet.2010.12.016
Annotation	The eukaryotic-type serine/threonine kinase StkP from Streptococcus pneumoniae is an important signal-transduction element that regulates the expression of numerous pneumococcal genes. We have expressed the extracellular C-terminal domain of StkP kinase (C-StkP), elaborated a three-dimensional structural model and performed a spectroscopical characterization of its structure and stability. Biophysical experiments show that C-StkP binds to synthetic samples of the cell wall peptidoglycan (PGN) and to beta-lactam antibiotics, which mimic the terminal portions of the PGN stem peptide. This is the first experimental report on the recognition of a minimal PGN unit by a PASTA-containing kinase, suggesting that non-crosslinked PGN may act as a signal for StkP function and pointing to this protein as an interesting target for beta-lactam antibiotics.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2012

Number of the records: 1