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Amino Acid Analysis. Methods and Protocols
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SYSNO ASEP 0369909 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Heptafluorobutyl chloroformate - based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography Author(s) Šimek, Petr (BC-A) RID, ORCID
Hušek, Petr (BC-A)
Zahradníčková, Helena (BC-A) RID, ORCIDNumber of authors 3 Source Title Amino Acid Analysis. Methods and Protocols, 828 - Methods in Molecular Biology. - New York : Springer Science & Business Media, 2012 / Alterman M. A. ; Hunziker P. - ISBN 978-1-61779-444-5 Pages s. 137-152 Number of pages 16 s. Number of copy 500 Number of pages 363 Language eng - English Country US - United States Keywords amino acid ; serum ; plasma Subject RIV CB - Analytical Chemistry, Separation R&D Projects 7F09028 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NS9755 GA MZd - Ministry of Health (MZ) GA203/09/2014 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000321876500014 DOI 10.1007/978-1-61779-445-2_13 Annotation Gas chromatography (GC) is a commonly used technique in amino acid analysis (AAA). However, one of the requirements of the application of GC for AAA is a need for the polar analytes to be converted into their volatile, thermally stable derivatives. In the last two decades, alkyl chloroformates have become attractive derivatization reagents. The reagents react immediately with most amino acid functional groups in aqueous matrices and the process can easily be coupled with liquid–liquid extraction of the resulting less-polar derivatives into immiscible organic phase. Here, we describe a simple protocol for in situ derivatization of amino acids with heptafluorobutyl chloroformate followed by subsequent chiral as well as nonchiral GC/mass spectrometric analysis on a respective nonpolar fused silica and an enantioselective Chirasil-Val capillary column. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2012
Number of the records: 1