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Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
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SYSNO ASEP 0369489 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy Author(s) Michálek, Jan (FGU-C) RID
Čapek, Martin (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCIDSource Title Microscopy Research and Technique - ISSN 1059-910X
Roč. 74, č. 9 (2011), s. 831-838Number of pages 8 s. Language eng - English Country US - United States Keywords confocal laser scanning microscopy ; image enhancement ; morphology filters Subject RIV JC - Computer Hardware ; Software R&D Projects GA102/08/0691 GA ČR - Czech Science Foundation (CSF) GA304/09/0733 GA ČR - Czech Science Foundation (CSF) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ME09010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000294401200007 DOI 10.1002/jemt.20965 Annotation In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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