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Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata
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SYSNO ASEP 0368586 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata Author(s) Molnár, I. (HU)
Kubaláková, Marie (UEB-Q) RID
Šimková, Hana (UEB-Q) RID, ORCID
Cseh, A. (HU)
Molnár-Láng, M. (HU)
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 66, č. 11 (2011), s. 1-11Number of pages 11 s. Language eng - English Country US - United States Keywords wheat ; chromosome isolation ; Aegilops umbellulata Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08025 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000298162000035 DOI 10.1371/journal.pone.0027708 Annotation This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted chromosomes, thus confirming the usefulness of this approach for physical mapping. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2012
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