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Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin
- 1.0368520 - ÚEB 2012 RIV NL eng J - Journal Article
Klemš, M. - Slámová, Z. - Motyka, Václav - Malbeck, Jiří - Trávníčková, Alena - Macháčková, Ivana - Holík, Josef - Procházka, S.
Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin.
Plant Growth Regulation. Roč. 65, č. 3 (2011), s. 427-437. ISSN 0167-6903. E-ISSN 1573-5087
R&D Projects: GA MŠMT 1M06030; GA ČR(CZ) GAP506/11/0774; GA AV ČR IAA600380701
Institutional research plan: CEZ:AV0Z50380511
Keywords : Beta-glucosidase * Cytokinin oxidase/ dehydrogenase * Dihydrozeatin
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.604, year: 2011
The uptake and metabolism of trans-zeatin (Z) and/or dihydrozeatin (DHZ), in correlation with cytokinin oxidase/dehydrogenase (CKX) and glucosidase activity, were studied in leaf segments derived from wild-type (WT) and transgenic (T) tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) during in vitro induction of shoot organogenesis. T explants harbored the maize gene Zm-p60.1-glucosidase. Higher levels of shoot regeneration were observed on T explants in the early stages of cultivation. In WT explants, the content of cytokinin (CK)-O- and N-glucosides increased. In T explants, a higher content of Z-9-riboside and its monophosphate and higher CKX activity during the early stage of cultures were found. A positive correlation was obtained for bioactive CK content and the organogenic response in T explants. Our results indicate a connection between the organogenic capacity of tobacco explants, metabolism of endogenous CKs and uptake of exogenous CKs from the cultivation medium.
Permanent Link: http://hdl.handle.net/11104/0202845
Number of the records: 1