Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

Norris, S. C. P.; Humpolíčková, Jana; Amler, Evžen; Huranová, Martina; Buzgo, Matej; Macháň, Radek; Lukáš, D.; Hof, Martin

doi:https://dx.doi.org/10.1016/j.actbio.2011.07.012

Number of the records: 1

Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds

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SYSNO ASEP	0368065
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds
Author(s)	Norris, S. C. P. (US) Humpolíčková, Jana (UFCH-W)_RID Amler, Evžen (UEM-P)_RID Huranová, Martina (UMG-J)_ORCID Buzgo, Matej (UEM-P) Macháň, Radek (UFCH-W) Lukáš, D. (CZ) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Acta Biomaterialia. - : Elsevier - ISSN 1742-7061 Roč. 7, č. 12 (2011), s. 4195-4203
Number of pages	9 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	fluorecence dynamics ; transient binding ; diffusion
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA500390702 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GAP304/10/1307 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
	AV0Z50390512 - UEM-P (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000297436500012
DOI	10.1016/j.actbio.2011.07.012
Annotation	Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2012

Number of the records: 1