DNA damage and nucleotide excision repair capacity in healthy individuals

0367811 - ÚEM 2012 RIV US eng J - Journal Article
Slyšková, Jana - Naccarati, Alessio - Poláková, Veronika - Pardini, Barbara - Vodičková, Ludmila - Štětina, R. - Schmuczerová, Jana - Šmerhovský, Z. - Lipská, L. - Vodička, Pavel
DNA damage and nucleotide excision repair capacity in healthy individuals.
Environmental and Molecular Mutagenesis. Roč. 25, č. 7 (2011), s. 511-517. ISSN 0893-6692. E-ISSN 1098-2280
R&D Projects: GA ČR GAP304/10/1286; GA MŠMT 7F10069
Grant - others:GA MŠk(CZ) GAUK124710
Institutional research plan: CEZ:AV0Z50390512
Keywords : BPDE-induced DNA repair capacity * comet assay * interindividual variability
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.709, year: 2011

Interindividual differences in DNA repair capacity (DRC) represent an important source of variability in genome integrity and thus influence health risk. DRC represents as well a potential biomarker in cancer prediction. The ultimate goal of our study was to obtain reference data from cancer-free population, e.g. background level for further investigations on cancer patients. Endogenous damage and DRC, specific for nucleotide excision repair (NER), was evaluated using modified comet assay and using challenge assay, genetic polymorphisms in NER genes were assessed as well. DNA damage and DRC exerted substantial interindividual variability, were associated with several confounders and modulated by particular genotypes of NER genes.
Permanent Link: http://hdl.handle.net/11104/0202358