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Glutathione peroxidase activity in the selenium-treated alga Scenedesmus quadricauda
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SYSNO ASEP 0367412 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Glutathione peroxidase activity in the selenium-treated alga Scenedesmus quadricauda Author(s) Vítová, Milada (MBU-M) RID, ORCID
Bišová, Kateřina (MBU-M) RID
Hlavová, Monika (MBU-M)
Zachleder, Vilém (MBU-M) ORCID
Rucki, M. (CZ)
Čížková, Mária (MBU-M) ORCIDSource Title Aquatic Toxicology. - : Elsevier - ISSN 0166-445X
Roč. 102, 1-2 (2011), s. 87-94Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords Cell cycle ; Enzyme activity ; Glutathione peroxidase Subject RIV EE - Microbiology, Virology R&D Projects GA525/09/0102 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000289743700011 DOI 10.1016/j.aquatox.2011.01.003 Annotation The green alga Scenedesmus quadricauda was used to investigate the relationship between the toxicity of selenium and the glutathione peroxidase activity. Selenium resistant strains SeIV and SeVI were synchronized and grown in high concentrations of Se (selenite or selenate). As a measure of selenium toxicity the EC50 values were determined. During growth of the untreated wild type, glutathione peroxidase activity increased slightly and then declined gradually until the end of the cell cycle. A similar pattern was observed in untreated resistant strains and when resistant strains were grown in the presence of selenium in the oxidation state to which they were resistant. In the wild type cultivated with 50 mg Se L−1 (selenite or selenate), activity increased to a high level and slowly declined until the end of the cell cycle Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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