Generation of two modified mouse alleles of the Hic1 tumor suppressor gene

0365113 - ÚMG 2012 RIV US eng J - Journal Article
Pospíchalová, Vendula - Turečková, Jolana - Fafílek, Bohumil - Vojtěchová, Martina - Krausová, Michaela - Lukáš, Jan - Šloncová, Eva - Takacova, S. - Divoký, V. - Leprince, D. - Plachý, Jiří - Kořínek, Vladimír
Generation of two modified mouse alleles of the Hic1 tumor suppressor gene.
Genesis. Roč. 49, č. 3 (2011), s. 142-151. ISSN 1526-954X. E-ISSN 1526-968X
R&D Projects: GA ČR(CZ) GA204/07/1567; GA ČR(CZ) GD204/09/H058
Institutional research plan: CEZ:AV0Z50520514
Keywords : Hypermethylated In Cancer 1 * Hic1 tumor suppressor * gene targeting
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.527, year: 2011

HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine "reporter'' mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence.
Permanent Link: http://hdl.handle.net/11104/0200434