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Frequency of aneuploidy related to age in porcine oocytes
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SYSNO ASEP 0365035 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Frequency of aneuploidy related to age in porcine oocytes Author(s) Horňák, M. (CZ)
Jeseta, M. (CZ)
Musilová, P. (CZ)
Pavlok, Antonín (UZFG-Y) RID
Kubelka, Michal (UZFG-Y) RID, ORCID
Motlík, Jan (UZFG-Y) RID, ORCID
Rubeš, J. (CZ)
Anger, Martin (UZFG-Y) ORCIDNumber of authors 8 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 4 (2011), s. 1-5Number of pages 5 s. Language eng - English Country US - United States Keywords porcine ; oocytes ; aneuploidy Subject RIV EE - Microbiology, Virology R&D Projects GA523/09/0743 GA ČR - Czech Science Foundation (CSF) IAA501620801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000290019400013 DOI 10.1371/journal.pone.0018892 Annotation It is generally accepted that mammalian oocytes are frequently suffering from chromosome segregation errors during meiosis I, which have severe consequences, including pregnancy loss, developmental disorders and mental retardation. In a search for physiologically more relevant model than rodent oocytes to study this phenomenon, we have employed comparative genomic hybridization (CGH), combined with whole genome amplification (WGA), to study the frequency of aneuploidy in porcine oocytes, including rare cells obtained from aged animals. Using this method, we were able to analyze segregation pattern of each individual chromosome during meiosis I. In contrast to the previous reports where conventional methods, such as chromosome spreads or FISH, were used to estimate frequency of aneuploidy, our results presented here show, that the frequency of this phenomenon was overestimated in porcine oocytes. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2012
Number of the records: 1