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Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium
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SYSNO ASEP 0362853 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium Author(s) Řezanka, Tomáš (MBU-M) ORCID
Siřišťová, L. (CZ)
Matoulková, D. (CZ)
Sigler, Karel (MBU-M) RIDSource Title Lipids. - : Wiley - ISSN 0024-4201
Roč. 46, č. 8 (2011), 765-780Number of pages 16 s. Language eng - English Country US - United States Keywords Plasmalogens ; Pectinatus ; Liquid chromatography-electrospray tandem mass spectrometry Subject RIV EE - Microbiology, Virology R&D Projects 1M0570 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000292568000009 DOI 10.1007/s11745-011-3556-y Annotation Liquid chromatography–electrospray tandem mass spectrometry (LC/ESI–MS/MS) was used to analyze phospholipids from three species of the anaerobic beerspoilage bacterial genus Pectinatus. Analysis of total lipids by HILIC (Hydrophilic Interaction Liquid Chromatography) column succeeded in separating diacyl- and plasmalogen phospholipids. Plasmalogens were then analyzed by means of the ESI–MS/MS and more than 220 molecular species of four classes of plasmalogens (PlsCho (choline plasmalogen), PlsEtn (ethanolamine plasmalogen), PlsGro (glycerol plasmalogen), and PlsSer (serine plasmalogen)) were identified. Major molecular species were c-p19:0/15:0 PlsEtn and PlsSer, which accounted for more than 4 percent of the total lipids Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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