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Investigating the early stages of Photosystem II assembly in Synechocystis sp. PCC 6803: isolation of CP47 and CP43 complexe
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SYSNO ASEP 0362744 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Investigating the early stages of Photosystem II assembly in Synechocystis sp. PCC 6803: isolation of CP47 and CP43 complexe Author(s) Boehm, M. (GB)
Romero, E. (NL)
Reisinger, V. (NO)
Yu, J. (GB)
Komenda, Josef (MBU-M) RID, ORCID
Eichacker, L. A. (NO)
Dekker, J. P. (NL)
Nixon, P. J. (GB)Source Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 286, č. 17 (2011), 14812-14819Number of pages 8 s. Language eng - English Country US - United States Keywords ENERGY CHLOROPHYLL STATES ; ANTENNA PROTEIN COMPLEX ; OXYGEN-EVOLVING CENTER Subject RIV EE - Microbiology, Virology R&D Projects IAA400200801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000289788300011 DOI 10.1074/jbc.M110.207944 Annotation Using the cyanobacterium Synechocystis sp. PCC 6803, we describe here the isolation of the CP47 and CP43 subunits, which, during biogenesis, attach to a reaction center assembly complex containing D1, D2, and cytochrome b559, with CP47 binding first. Absorption spectroscopy and pigment analyses revealed that both CP47-His and CP43-His bind chlorophyll a and âcarotene. A comparison of the low temperature absorption and fluorescence spectra in theQY region for CP47-His and CP43-His with those for CP47 and CP43 isolated by fragmentation of spinach PSII core complexes confirmed that the spectroscopic properties are similar but not identical. Immunoblotting and mass spectrometry revealed the co-purification of PsbH, PsbL, and PsbT with CP47-His and of PsbK and Psb30/Ycf12 with CP43-His. Overall, our data support the view that CP47 and CP43 form preassembled pigment-protein complexes in vivo before their incorporation into the PSII complex Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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