Number of the records: 1
Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method
- 1.
SYSNO ASEP 0361674 Document Type P - Patent R&D Document Type Patent or other outcome protected by special legislation Title Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method Author(s) Pytlík, R. (CZ)
Hofman, P. (CZ)
Trč, T. (CZ)
Stehlík, D. (CZ)
Soukup, T. (CZ)
Kobylka, P. (CZ)
Klener, P. (CZ)
Rypáček, František (UMCH-V) RID
Mulinková, Katarína (UMCH-V)Year of issue 2011 Possible third party use of the result A - Pro využití výsledku jiným subjektem je vždy nutné nabytí licence Royalty requested A - Poskytovatel licence požaduje licenční poplatek Patent no. or utility model no. or industrial design no. EP1999250 Date of the patent acceptance 20.07.2011 Name of the patent owner Univerzita Karlova v Praze - Ústav hematologie a krevní transfuze - Ústav makromolekulární chemie AV ČR, v. v. i. Code of the issuer name EPO_1 - European Patent Office Munich, The Hague, Berlin, Vienna, Brusselshttp://www.epo.org/ Current use A - Pouze udělený (dosud nevyužívaný) patent nebo patent využívaný jeho vlastníkem Language eng - English Keywords mesenchymal stem cells ; cultivation ; bone-fracture treatment Subject RIV FJ - Surgery incl. Transplants R&D Projects ND7448 GA MZd - Ministry of Health (MZ) CEZ AV0Z40500505 - UMCH-V (2005-2011) Annotation The invention relates to novel method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, the said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for about one to three weeks in CellGroTM Hematopoietic Stem cell Medium, certified for clinical use, with addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under standard conditions for the cultivation of tissue culture. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012 Electronic address http://worldwide.espacenet.com/publicationDetails/originalDocument?FT=D&date=20110720&DB=EPODOC&locale=en_EP&CC=EP&NR=1999250B1&KC=B1
Number of the records: 1