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Polymerase construction of base-modified DNA for chemical biology
- 1.0361521 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Hocek, Michal - Macíčková-Cahová, Hana - Kielkowski, Pavel - Raindlová, Veronika - Kalachová, Lubica - Riedl, Jan - Balintová, Jana - Ménová, Petra
Polymerase construction of base-modified DNA for chemical biology.
Chemistry of Nucleic Acid Components. 15th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i., 2011 - (Hocek, M.), s. 152-154. Collection Symposium Series, 12. ISBN 978-80-86241-37-1.
[Chemistry of Nucleic Acid Components /15./. Český Krumlov (CZ), 05.06.2011-10.06.2011]
R&D Projects: GA MŠMT LC512; GA AV ČR(CZ) IAA400040901; GA ČR GA203/09/0317
Institutional research plan: CEZ:AV0Z40550506
Keywords : DNA * polymerases * nucleotides
Subject RIV: CC - Organic Chemistry
An efficient two-step methodology for construction of base-modified DNA was developed based on aqueous-phase cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) followed by polymerase incorporation. A number of diverse chemical modifications have been successfully incorporated in this way for the use in chemical biology (modulation of cleavage by restriction enzymes, interactions with diverse DNA-binding proteins and bioconjugations).
Permanent Link: http://hdl.handle.net/11104/0198819
Number of the records: 1