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Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged 3 antigens: Preparation, structural study and immune response towards rHsp90
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SYSNO ASEP 0361499 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged 3 antigens: Preparation, structural study and immune response towards rHsp90 Author(s) Mašek, J. (CZ)
Bartheldyová, E. (CZ)
Turánek-Knotigová, P. (CZ)
Škrabalová, M. (CZ)
Korvasová, Z. (CZ)
Plocková, J. (CZ)
Koudelka, Š. (CZ)
Škodová, P. (CZ)
Kulich, P. (CZ)
Křupka, M. (CZ)
Zachová, K. (CZ)
Czerneková, L. (CZ)
Horynová, M. (CZ)
Kratochvílová, Irena (FZU-D) RID, ORCID, SAI
Miller, A. D. (GB)
Zyka, Daniel (UOCHB-X) RID
Michálek, J. (CZ)
Vrbová, J. (CZ)
Šebela, M. (CZ)
Ledvina, Miroslav (UOCHB-X) RID
Raška, M. (CZ)
Turánek, J. (CZ)Source Title Journal of Controlled Release. - : Elsevier - ISSN 0168-3659
Roč. 151, č. 2 (2011), s. 193-201Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords recombinant vaccine ; liposome Subject RIV EC - Immunology R&D Projects GAP304/10/1951 GA ČR - Czech Science Foundation (CSF) KAN200520703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z10100520 - FZU-D (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000291845600012 DOI 10.1016/j.jconrel.2011.01.016 Annotation Hsp90-CA is present in cell wall of Candida pseudohyphae or hyphae - typical pathogenic morphotype for 31 both systemic and mucosal Candida infections. Heat shock protein from Candida albicans (hsp90-CA) is an 32 important target for protective antibodies during disseminated candidiasis of experimental mice and human. 33 His-tagged protein rHsp90 was prepared and used as the antigen for preparation of experimental 34 recombinant liposomal vaccine. Nickel-chelating liposomes (the size around 100 nm, PDI≤0.1) were 35 prepared from the mixture of egg phosphatidyl choline and nickel-chelating lipid DOGS-NTA-Ni (molar ratio 36 95:5%) by hydration of lipid film and extrusion methods. New non-pyrogenic hydrophobised derivative of 37 MDP (C18-O-6-norAbuMDP) was incorporated into liposomes as adjuvans. by metallochelating bond and the structure of these proteoliposomes 39 was studied by dynamic light scattering, AF microscopy, TEM and GPC. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2012
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