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Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase
- 1.0360800 - MBÚ 2012 RIV GB eng J - Journal Article
Grulich, Michal - Maršálek, Jaroslav - Kyslík, Pavel - Štěpánek, Václav - Kotík, Michael
Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase.
Process Biochemistry. Roč. 46, č. 2 (2011), s. 526-532. ISSN 1359-5113. E-ISSN 1873-3298
R&D Projects: GA ČR GAP207/10/0135; GA ČR GAP504/10/0137
Institutional research plan: CEZ:AV0Z50200510
Keywords : Overexpression * Fed-batch * Aqueous two-phase system
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.627, year: 2011
Optimised culture conditions for the recombinant prototrophic strain Escherichia coli RE3(pSEKau2) were established to obtain biomass of epoxide hydrolase (EH). The recombinant enzyme was extracted from crude extracts using an optimised polyethylene glycol-based aqueous two-phase system (ATPS). Eupergit C 250L carrier proved to be the most interesting in terms of partially purified EH immobilization. The immobilized EH exhibited a significantly higher thermal stability compared to the free enzyme. Immobilization did not alter, to a large extent, the enantioselectivity and regioselectivity of the enzyme
Permanent Link: http://hdl.handle.net/11104/0198266
Number of the records: 1