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Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
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SYSNO ASEP 0360791 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber Author(s) Siřišťová, L. (CZ)
Luhový, R. (CZ)
Sigler, Karel (MBU-M) RID
Řezanka, Tomáš (MBU-M) ORCIDSource Title Extremophiles. - : Springer - ISSN 1431-0651
Roč. 15, č. 3 (2011), 423-429Number of pages 7 s. Language eng - English Country JP - Japan Keywords Thermophilic bacteria ; Geobacillus ; Meiothermus Subject RIV EE - Microbiology, Virology CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000290037700012 DOI 10.1007/s00792-011-0373-4 Annotation Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
Number of the records: 1