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Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering
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SYSNO ASEP 0360755 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering Author(s) Horák, Daniel (UMCH-V) RID, ORCID
Matulka, K. (CZ)
Hlídková, Helena (UMCH-V) RID, ORCID
Lapčíková, Monika (UMCH-V) RID
Beneš, Milan J. (UMCH-V) RID
Jaroš, J. (CZ)
Hampl, Aleš (UEM-P)
Dvořák, Petr (UEM-P)Source Title Journal of Biomedical Materials Research. Part B. - : Wiley - ISSN 1552-4973
98B, č. 1 (2011), s. 54-67Number of pages 14 s. Language eng - English Country US - United States Keywords poly(N,N-diethylacrylamide) ; porosity ; YIGSR peptide Subject RIV FH - Neurology R&D Projects 1M0538 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP304/11/0731 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40500505 - UMCH-V (2005-2011) AV0Z50390512 - UEM-P (2005-2011) AV0Z50390703 - UEM-P (2007-2013) UT WOS 000291598900007 DOI 10.1002/jbm.b.31832 Annotation Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012
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