Number of the records: 1  

Genome mining for the discovery of new nitrilases in filamentous fungi

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    SYSNO ASEP0360696
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleGenome mining for the discovery of new nitrilases in filamentous fungi
    Author(s) Kaplan, Ondřej (MBU-M)
    Bezouška, Karel (MBU-M)
    Malandra, Anna (MBU-M) ORCID
    Veselá, Alicja Barbara (MBU-M) RID
    Petříčková, Alena (MBU-M) RID
    Felsberg, Jürgen (MBU-M)
    Rinágelová, Anna (MBU-M)
    Křen, Vladimír (MBU-M) RID, ORCID
    Martínková, Ludmila (MBU-M) RID, ORCID
    Source TitleBiotechnology Letters. - : Springer - ISSN 0141-5492
    Roč. 33, č. 2 (2011), 309-312
    Number of pages4 s.
    Languageeng - English
    CountryNL - Netherlands
    KeywordsAromatic nitrilase ; Arylacetonitrilase ; Aspergillus niger
    Subject RIVEE - Microbiology, Virology
    R&D ProjectsIAA500200708 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    OC09046 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    FT-TA5/043 GA MPO - Ministry of Industry and Trade (MPO)
    GD305/09/H008 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z50200510 - MBU-M (2005-2011)
    UT WOS000286198700014
    DOI10.1007/s10529-010-0421-7
    AnnotationOur aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an aktivity of 390 U l culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l culture, respectively, with phenylacetonitrile as substrate
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2012
Number of the records: 1  

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