Genome mining for the discovery of new nitrilases in filamentous fungi

Kaplan, Ondřej; Bezouška, Karel; Malandra, Anna; Veselá, Alicja Barbara; Petříčková, Alena; Felsberg, Jürgen; Rinágelová, Anna; Křen, Vladimír; Martínková, Ludmila

doi:https://dx.doi.org/10.1007/s10529-010-0421-7

Number of the records: 1

Genome mining for the discovery of new nitrilases in filamentous fungi

1.

SYSNO ASEP	0360696
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Genome mining for the discovery of new nitrilases in filamentous fungi
Author(s)	Kaplan, Ondřej (MBU-M) Bezouška, Karel (MBU-M) Malandra, Anna (MBU-M)_ORCID Veselá, Alicja Barbara (MBU-M)_RID Petříčková, Alena (MBU-M)_RID Felsberg, Jürgen (MBU-M) Rinágelová, Anna (MBU-M) Křen, Vladimír (MBU-M)_{RID, ORCID} Martínková, Ludmila (MBU-M)_{RID, ORCID}
Source Title	Biotechnology Letters. - : Springer - ISSN 0141-5492 Roč. 33, č. 2 (2011), 309-312
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Aromatic nitrilase ; Arylacetonitrilase ; Aspergillus niger
Subject RIV	EE - Microbiology, Virology
R&D Projects	IAA500200708 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC09046 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	FT-TA5/043 GA MPO - Ministry of Industry and Trade (MPO)
	GD305/09/H008 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000286198700014
DOI	10.1007/s10529-010-0421-7
Annotation	Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an aktivity of 390 U l culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l culture, respectively, with phenylacetonitrile as substrate
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2012

Number of the records: 1