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2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential
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SYSNO ASEP 0360024 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential Author(s) Matoušová, Marika (UOCHB-X) ORCID
Votruba, Ivan (UOCHB-X) RID
Otmar, Miroslav (UOCHB-X) RID
Tloušťová, Eva (UOCHB-X) RID, ORCID
Günterová, Jana (UOCHB-X) RID, ORCID
Mertlíková-Kaiserová, Helena (UOCHB-X) RID, ORCIDNumber of authors 6 Source Title Epigenetics. - : Taylor & Francis - ISSN 1559-2294
Roč. 6, č. 6 (2011), s. 769-776Number of pages 8 s. Language eng - English Country US - United States Keywords epigenetic therapy ; nucleoside analogs ; DNA methylation ; 2'-deoxy-5-azacytidine ; 2'-deoxy-5,6-dihydro-5-azacytidine Subject RIV CE - Biochemistry R&D Projects 1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000291148100013 DOI 10.4161/epi.6.6.16215 Annotation A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2´-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2´-deoxy-5,6-dihydro-5-azacytidine and its α-anomer, and of a 2-pyrimidone ribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2´-deoxycytidine 5´-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2´-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2´-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2012
Number of the records: 1