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Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing
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SYSNO ASEP 0359988 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing Author(s) Koval, Dušan (UOCHB-X) RID, ORCID
Kašička, Václav (UOCHB-X) RID, ORCID
Cottet, H. (FR)Number of authors 3 Source Title Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 413, č. 1 (2011), s. 8-15Number of pages 8 s. Language eng - English Country US - United States Keywords capillary zone electrophoresis ; capillary isoelectric focusing ; glycated hemoglobin HbA1c Subject RIV CB - Analytical Chemistry, Separation R&D Projects GP203/09/P485 GA ČR - Czech Science Foundation (CSF) GA203/08/1428 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000289607900002 DOI 10.1016/j.ab.2011.01.048 Annotation Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2012
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