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Fluorescent magnetic nanoparticles for biomedical applications
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SYSNO ASEP 0359777 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fluorescent magnetic nanoparticles for biomedical applications Author(s) Chekina, Nataliya (UMCH-V) RID
Horák, Daniel (UMCH-V) RID, ORCID
Jendelová, Pavla (UEM-P) RID, ORCID
Trchová, Miroslava (UMCH-V) RID, ORCID
Beneš, Milan J. (UMCH-V) RID
Hrubý, Martin (UMCH-V) RID, ORCID
Herynek, V. (CZ)
Turnovcová, Karolína (UEM-P) ORCID
Syková, Eva (UEM-P) RIDSource Title Journal of Materials Chemistry. - : ROYAL SOC CHEMISTRY - ISSN 0959-9428
Roč. 21, č. 21 (2011), s. 7630-7639Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords magnetic ; fluorescent ; stem cells Subject RIV CD - Macromolecular Chemistry R&D Projects 7E09109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA203/09/1242 GA ČR - Czech Science Foundation (CSF) KAN201110651 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KAN200200651 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40500505 - UMCH-V (2005-2011) AV0Z50390703 - UEM-P (2007-2013) UT WOS 000290670000017 DOI 10.1039/C1JM10621J Annotation The simultaneous combination of optical and magnetic resonance imaging (MRI) would greatly benefit in vivo disease diagnosis as well as in situ monitoring of living cells. In order to design dual detection of cells involving simultaneous imaging by fluorescent microscopy and MRI, nanoparticles with two reporters, a fluorescent dye and a superparamagnetic core, included in one particle were synthesized and characterized. The γ-Fe2O3 nanoparticles obtained by coprecipitation and oxidation were coated with silica (SiO2) or carboxymethyl chitosan (CMCS) and labeled with fluorescein isothiocyanate (FITC). The fluorescent label was covalently bound to the nanoparticles and was not quenched by the iron oxide core. The nanoparticles successfully labeled rat mesenchymal stem cells (rMSCs) in vitro. Relaxation time measurements found large amounts of iron inside the cells with FITC-labeled γ-Fe2O3–SiO2-AP nanoparticles. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012
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