Number of the records: 1
Serum/Plasma Proteomics Metods and Protocols
- 1.
SYSNO ASEP 0359480 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Intact protein separation by 1 and 2dimension liquid chromatography for the comparative proteomic separation of partitioned serum or plasma Author(s) Sheng, S. (US)
Skalníková, Helena (UZFG-Y) RID, ORCID
Meng, A. (US)
Fu, Q. (CN)
Everett, A. (US)
Van Eyk, J. (US)Source Title Serum/Plasma Proteomics Metods and Protocols. - NEW YORK : Humana Press, 2011 - ISBN 978-1-61779-067-6 Pages s. 29-46 Number of pages 17 s. Number of copy 300 Number of pages 397 Language eng - English Country US - United States Keywords liquid chromatography ; chromatofocusing ; reversed phase Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000289957500002 Annotation Serum and plasma-based biomarker discovery requires technologies with specific capabilities: sufficient proteome coverage and depth; technical reproducibly; and the scalability to enable analysis on substantial volumes of a large number of samples at reasonable cost. We have shown that plasma samples processed using ProteomeLab™ IgY-12 LC10 Proteome Partitioning Kits to selectively remove the 12 most highly-abundant proteins, followed by intact protein separation by two-dimensional liquid chromatography (2DLC, chromatofocusing and reversed phase) can unique enrich for middle –lower-abundant proteins. Equally, 1DLC (reversed phase) separation of intact proteins is complementary to 2DLC. The serial use of a single piece of equipment can be prohibitively time-consuming and thus, this chapter also describes the harmonization of multiple LC instruments in order to minimize technical variation and ensure reproducibility. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2012
Number of the records: 1