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Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
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SYSNO ASEP 0358220 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53 Author(s) Šípová, Hana (URE-Y)
Špringer, Tomáš (URE-Y)
Homola, Jiří (URE-Y) RIDNumber of authors 3 Source Title Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 399, č. 7 (2011), s. 2343-2350Number of pages 8 s. Language eng - English Country DE - Germany Keywords Surface Plasmon Resonance ; point mutation ; amplification Subject RIV JB - Sensors, Measurment, Regulation R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) OC09058 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA202/09/0193 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z20670512 - URE-Y (2005-2011) UT WOS 000287141700006 DOI 10.1007/s00216-010-3863-9 Annotation This paper reports an approach to detection of single nucleotide polymorphism based on special amplification assay and surface plasmon resonance (SPR) biosensor technology. In this assay, a part of the target DNA is recognized by a probe coupled with streptavidin-oligonucleotide (SON) complexes ex-situ. The SON-target complex is then detected with the SPR sensor with high specificity and sensitivity. The assay is applied to detection of short analogue of TP53 at levels down to 40 pM. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2011
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