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The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva
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SYSNO ASEP 0357702 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva Author(s) Merjava, S. (CZ)
Neuwirth, Aleš (UMG-J) ORCID
Tanzerova, M. (CZ)
Jirsová, K. (CZ)Source Title Histology and Histopathology. - : Universidad de Murcia - ISSN 0213-3911
Roč. 26, č. 3 (2011), s. 323-331Number of pages 9 s. Language eng - English Country ES - Spain Keywords Posterior polymorphous dystrophy ; Immunohostochemical analysis ; Impression cytology Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000285921200005 Annotation The most intense staining present throughout the cornea was observed for CK3, CK5 and CK14; CK19 was found at the corneal periphery only. CK4 and CK10/13 revealed mild to moderate positivity mostly in the superficial layers of the cornea. The suprabasal cell layers of all examined areas showed a strong positivity for CK16. A heterogeneous staining pattern with a centrifugal decrease in the signal was observed for CK8 and CK18. CK5/6, CK14 and CK19 were present in the limbus, where a positive signal for CK3 was observed in the suprabasal and superficial cells only. In contrast to the cornea, CK15 appeared in the basal and suprabasal layers of the limbus. The perilimbal conjunctiva showed strong immunostaining for CK10/13, CK14 and CK19. A moderate signal for CK7 was detected in the superficial layers of the conjunctiva. qRT-PCR confirmed CK6 and CK18 expression in the corneal and conjunctival epithelium. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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