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Enzymatic Reaction – Fractionation Process under SC-CO2 as a Way to Concentrate DHA from Hake Skin Oil
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SYSNO ASEP 0356221 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Enzymatic Reaction – Fractionation Process under SC-CO2 as a Way to Concentrate DHA from Hake Skin Oil Author(s) Rubio-Rodrígueza, N. (ES)
Rochová, Kristina (UCHP-M)
Sovová, Helena (UCHP-M) RID, ORCID, SAI
Beltrána, S. (ES)Source Title Abstracts. - Graz : -, 2010 - ISBN 978-2905267-72-6 Pages s. 101 Number of pages 1 s. Publication form CD-ROM - CD-ROM Action European Meeting on Supercritical Fluids /12./ Event date 09.05.2010-12.05.2010 VEvent location Graz Country AT - Austria Event type WRD Language eng - English Country AT - Austria Keywords omega-3 concentrates ; enzymatic reaction ; supercritical CO2 Subject RIV CI - Industrial Chemistry, Chemical Engineering CEZ AV0Z40720504 - UCHP-M (2005-2011) Annotation A continuous enzymatic hydrolysis–fractionation process under SC-CO2 is proposed as a method to concentrate DHA, as acylglyceride, from hake oil. To evaluate the reaction kinetics, hydrolysis experiments were carried out with a sn-1,3 specific lipase from Mucor miehi immobilized on a macroporous ion-exchange resin at 313 K, pressures and CO2 flow rates (0.5-1.9 g/min). Fixing the reaction conditions at 313 K, 25 MPa and 0.5 g CO2/min, a subsequent fractionation in two separators S1 and S2 in series was studied changing pressure (8.3-25 MPa) and temperature (308-333 K) in S1. The best separation factors between acylglyceride and FFA, and DHA and palmitic acid respectively, were obtained working in S1 at a density close to the critical density of CO2. DHA was slightly concentrated as acylglyceride S1. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2011
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