Laser Desorption-Ionization of Lipid Transfers: Tissue Mass Spectrometry Imaging without MALDI Matrix

0354297 - MBÚ 2011 RIV US eng J - Journal Article
Vidová, V. - Novák, Petr - Strohalm, Martin - Pól, Jaroslav - Havlíček, Vladimír - Volný, Michael
Laser Desorption-Ionization of Lipid Transfers: Tissue Mass Spectrometry Imaging without MALDI Matrix.
Analytical Chemistry. Roč. 82, č. 12 (2010), s. 4994-4997. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA MŠMT LC545; GA MŠMT LC07017; GA ČR GPP206/10/P018
Institutional research plan: CEZ:AV0Z50200510
Keywords : ELECTROSPRAY-IONIZATION * METABOLITES * SECTIONS
Subject RIV: EE - Microbiology, Virology
Impact factor: 5.874, year: 2010

Mass spectrometry imaging of tissue-lipid transfers without MALDI matrix is demonstrated. Commercially available nanostructured surfaces (nano-assisted laser desorption-ionization or NALDI) are used as substrates for imprinting of tissue sections. The lithographic transfers are then washed and the two-dimensional distribution of the lipids is imaged by laser desorption-ionization mass spectrometry. The NALDI imaging of lipid transfers is compared with standard MALDI imaging of matrix-coated tissue sections. The obtained images are of the same quality, and no spatial information is lost due to the imprinting process. NALDI imaging is faster due to the absence of the time-consuming matrix deposition step, and the NALDI mass spectra are less complex and easier to interpret than standard MALDI
Permanent Link: http://hdl.handle.net/11104/0193334