IrML – a gene encoding a new member of the ML protein family from the hard tick, Ixodes ricinus

Horáčková, J.; Rudenko, Natalia; Golovchenko, Maryna; Havlíková, S.; Grubhoffer, Libor

doi:https://dx.doi.org/10.1111/j.1948-7134.2010.00100.x

Number of the records: 1

IrML – a gene encoding a new member of the ML protein family from the hard tick, Ixodes ricinus

1.

SYSNO ASEP	0353437
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	IrML – a gene encoding a new member of the ML protein family from the hard tick, Ixodes ricinus
Author(s)	Horáčková, J. (CZ) Rudenko, Natalia (BC-A)_{RID, ORCID} Golovchenko, Maryna (BC-A)_{RID, ORCID} Havlíková, S. (SK) Grubhoffer, Libor (BC-A)_{RID, ORCID}
Source Title	Journal of Vector Ecology - ISSN 1081-1710 Roč. 35, č. 2 (2010), s. 410-418
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	Ixodes ricinus ; tick ; ML-domain containing protein ; in situ hybridization ; gene expression ; ML (MD-2-related lipid-recognition) domain
Subject RIV	GJ - Animal Vermins ; Diseases, Veterinary Medicine
R&D Projects	GA524/06/1479 GA ČR - Czech Science Foundation (CSF)
	LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000285758200020
DOI	10.1111/j.1948-7134.2010.00100.x
Annotation	Blood intake causes significant changes in ticks, triggering vital physiological processes including differential gene expression. A gene encoding Ixodes ricinus ML-domain containing protein (IrML) is one of the set of the genes that are strongly induced by blood meals. IrML belongs to the ML protein family that commonly occurs in diverse organisms and is involved in lipid binding and transport, pathogen recognition or in immune response. An IrML gene was amplified from cDNA of engorged I. ricinus females using the gene-specific primers designed on a basis of partial sequences of related genes for ML domain protein. IrML was shown to be expressed mainly in the gut, but also in salivary glands and hemolymph of all tick developmental stages. Analysis of the predicted structure of I. ricinus ML-domain containing protein and its localization in the tick body could suggest that IrML is a secreted protein and is possibly involved in tick innate immunity.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1