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Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts usány chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry
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SYSNO ASEP 0352779 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts usány chromatographic fractionation followed by microcolumn liquid chromatography-mass spectrometry Author(s) Myjavcová, R. (CZ)
Marhol, Petr (MBU-M) RID
Křen, Vladimír (MBU-M) RID, ORCID
Šimánek, V. (CZ)
Ulrichová, J. (CZ)
Palíková, I. (CZ)
Papoušková, B. (CZ)
Lemr, Karel (MBU-M) RID, ORCID
Bednář, P. (CZ)Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1217, č. 51 (2010), s. 7932-7941Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords anthocyanin ; blueberry honeysuckle ; chromatography Subject RIV CB - Analytical Chemistry, Separation R&D Projects FT-TA3/024 GA MPO - Ministry of Industry and Trade (MPO) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000285429700002 DOI 10.1016/j.chroma.2010.05.058 Annotation Anthocyanins from the fruit Lonicera caerulea L. var. kamtschatica (blueberry honeysuckle, Caprifoliaceae) were studied via (semi)preparative chromatographic fractionation followed by MS and LC/MS analysis. The extraction procedure was optimized with respect to analytical purposes as well as its potential use for the preparation of nutraceuticals. The highest yield of anthocyanins was obtained using acidified methanol as the extraction medium. A comparable total anthocyanin content was obtained using a mixture of methanol and acetone. However, when Lonicera anthocyanins were in contact with acetone, a condensation reaction occurred to a large extent and related 5-methylpyranoanthocyanins were found. The effect of other extraction media, including ethanol as a “green” solvent, is also discussed Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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