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Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii
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SYSNO ASEP 0352314 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii Author(s) Margos, G. (GB)
Hojgaard, A. (US)
Lane, R. S. (US)
Cornet, M. (FR)
Fingerle, V. (DE)
Rudenko, Natalia (BC-A) RID, ORCID
Aanensen, D. M. (GB)
Fish, D. (US)
Piesman, J. (US)Source Title Ticks and Tick-borne Diseases - ISSN 1877-959X
Roč. 1, č. 4 (2010), s. 151-158Number of pages 8 s. Language eng - English Country DE - Germany Keywords Borrelia bissettii ; Borrelia kurtenbachii sp. nov. ; Ixodes ; Multilocus sequence analysis ; Molecular ecology Subject RIV EC - Immunology CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000286366600001 DOI 10.1016/j.ttbdis.2010.09.002 Annotation Using multilocus sequence analysis (MLSA), we investigated the phylogenetic relationship of spirochaete strains from North America previously assigned to the genospecies Borrelia bissettii.We amplified internal fragments of 8 housekeeping genes (clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA) located on the main linear chromosome by polymerase chain reaction. Phylogenetic analysis of concatenated sequences of the 8 loci showed that the B. bissettii clade consisted of 4 closely related clusters which included strains from California and Colorado that were isolated from Ixodes pacificus, I. spinipalpis, or infected reservoir hosts. Several strains isolated from I. scapularis clustered distantly from B. bissettii. Genetic distance analyses confirmed that these strains are more distant to B. bissettii than B. carolinensis, a recently described Borrelia species, which suggests that they constitute a new Borrelia genospecies, Borrelia kurtenbachii. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
Number of the records: 1